Occasionally, when bloods are tested with different anti-Rh (anti-D) sera, they give some but not all the serological reactions expected from the presence of the Rh (D) factor, or the intensity of such reactions is consistently weaker than the average. Such “intermediate variants” are usually symbolized as Du. Therefore, this test is usually performed to confirm the incomplete expression of Rh antigen in blood grouping. See also ABO & Rh Phenotyping and Genotyping by PCR
Specimen Preparation Do not freeze red cells.
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