Methotrexate, formerly known as amethopterin, is an antimetabolite and antifolate drug used in treatment of cancer and autoimmune diseases. It acts by inhibiting the metabolism of folic acid. This test is useful for monitoring treatment, establishing the correct dose and detecting evidence of toxicity.
Protect from light. Usual sampling time depends on dosage and clinical status of patient. Steady state achieved 12 - 24 Hrs after chronic dosing.
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