1. Sampling: ideally 3-4 hours after waking up (about 10:00-11:00 am), avoid any stress before sampling. 2. Fasting: no fastnig needed, a light breakfast is suggested 3. T4 therapy: take after blood sampling 4. Anti-thyroid drugs: no withdrawal 5. Biotin: stop 48 hrs before sampling (if dose > 5 mg/day)
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